PALM MicroBeam laser capture system
This core houses PALM MicroBeam with Laser Microdissection and Pressure Catapulting technology for noncontact and contamination-free microdissection and sample collection. The PALM MicroBeam incorporates lasers of high beam quality interfaced to a microscope and focused through objectives with high numerical aperture to a minimum possible spot size. Thus, processing accuracy under 1 μm can be reached, enabling the user to manipulate at the level of single cells or even subcellular components.
The cutting procedure is performed quickly and is devoid of heat transfer. Adjacent biological matter or biomolecules (i.e., DNA, RNA, or proteins) out of the focus areas are not affected. After the cutting procedure, the selected area is ejected from the object plane with a single laser pulse. The desired piece of interest is transported for several millimeters against gravity, directly into a capture device.