As a member of the Molecular Biology and Cancer Genetics team at the OSUCCC – James, Dr. Anand, along with his laboratory, has recently developed human adult stem cell (iPSC) derived models of cancer, autism and Alzheimer’s disease. In particular, Dr. Anand’s work focuses on understanding how genes and the environment give rise to such debilitating brain diseases.
Dr. Anand’s laboratory has independently generated human iPSC-derived synthetic neural organoids which exhibit a remarkable level of development that morphologically resembles a nearly complete human embryonic central nervous system at ~5 week in utero development, but after ~12 weeks in culture in vitro. Using a proprietary process, Dr. Anand and his team have engineered neural organoids from Alzheimer’s disease and tuberous sclerosis patient skin cells with identified genetic mutations in susceptibility genes. His lab’s transcriptomics results remarkably show comprehensive and accurate correlation of the dysregulated expression of hundreds of genes previously correlated with the clinical symptoms and/or pathologies of both of these diseases.
His team’s “NexGen” synthetic neural organoid models allow them to use whole genome transcriptomic to monitor changes in gene expression in response of the models to genetic or environmental perturbations of human brain physiology making their models useful for disease research and drug discovery efforts.
Arnold LE, Anand R, Aman M. (2013) Varenicline in autistic disorder: hypothesis and case report of single-patient crossover. J Child Adolesc Psychopharmacol 23:61-614.
Amici SA, McKay SB, Wells GB, Robson JI, Nasire M, Ponath G, Anand R. (2012) A highly conserved cytoplasmic cysteine residue in the alpha4 nicotinic acetylcholine receptor is palmitoylated and regulates protein expression. J Biol Chem 287:23119-23127.
Arnold LE, Aman MG, Holloway J, Hurt E, Bates B, Li X, Farmer C, Anand R, Thompson S, Ramadan Y, Williams C. (2012) Placebo-controlled pilot trial of mecamylamine for treatment of autism spectrum disorders. J Child Adolesc Psychopharmacology 22:198-205.
Mukherjee J, Kuryatov A, Moss SJ, Lindstrom JM, Anand R. (2009) Mutations of cytosolic loop residues impair assembly and maturation of alpha7 nicotinic acetylcholine receptors. J Neurochem 110:1885-1894.
Cheng SB, Amici SA, Ren XA, McKay SB, Treuil MW, Lindstrom JM, Rao J, Anand R. (2009) Presynaptic targeting of alpha4beta 2 nicotinic acetylcholine receptors is regulated by neurexin-1beta. J Biol Chem 284:23251-23259.
Zhao C, Anand R, Braunewell KH. (2009) Nicotine-induced Ca2+-myristoyl switch of neuronal Ca2+ sensor VILIP-1 in hippocampal neurons: a possible crosstalk mechanism for nicotinic receptors. Cell Mol Neurobiol 29:273-286.
Ruskin DN, Anand R, LaHoste GJ. (2008) Chronic menthol attenuates the effects of nicotine in adolescent but not adult rats. Nicotine Tob Res 10:1753-1759.
Ruskin DN, Anand R, LaHoste GJ. (2007) Menthol and nicotine oppositely modulate body temperature in the rat. Eur J Pharmacol 559:161-164.
Brackmann M, Schuchmann S, Anand R, Braunewell KH. (2005) Neuronal Ca++ sensor protein VILIP-1 affects cGMP signalling by regulating receptor recycling of guanylyl cyclase B in hippocampal neurons. J Cell Science 118:24895-2505.
Ren XQ, Cheng SB, Treuil MW, Mukherjee J, Rao J, Lindstrom JM, Anand R. (2005) Structural determinants of alpha4beta2 nicotinic acetylcholine receptor trafficking. J Neurosci 25:6676-6686.
Lin L, Jeanclos EM, Braunewell KH, Gundelfinger ED, Anand R. (2002) Functional analysis of calcium-binding EF-hand of Visinin-like protein-1. Biochem Biophy Res Comm 296:827-832.
Lin L, Jeanclos EM, Magdalen T, Braunewell KH, Gundelfinger ED, Anand R. (2002) The calcium sensor protein VILIP-1 modulates the surface expression and agonist-sensitivity of the nicotinic alpha4beta2 acetylcholine receptor. J Biol Chem 277:41872-41878.
MS: Indian Institute of Technology
PhD: The Ohio State University
Postdoctoral: Salk Institute, University of Pennsylvania