Assisting researchers with confocal imaging.

The Neuroscience Imaging Core provides neuroscience researchers with the training, expertise and equipment that they need to perform fluorescence confocal microscopy of cells, tissues and zebrafish embryos in the living and fixed state. We provide access to well-maintained wide-field, point-scanning and spinning disk confocal microscopes, as well as expert consultation and assistance with design and execution of imaging experiments.

Capabilities include support for long-term time-lapse imaging of cells, tissue slices and zebrafish embryos, advanced imaging applications such as imaging tiling, photoactivation, FRAP and FRET, 3D rendering, and sophisticated image processing and analysis capabilities.

Please see our Terms of Service and User Fee Structure for specifics regarding use of this core.


Andor Confocal

Andor Revolution WD Spinning Disk Confocal

The Andor Revolution WD is the Imaging Core’s spinning disk laser confocal system, ideal for live imaging of cells, tissues and zebrafish embryos. 

Features include:

  • Inverted Nikon TiE microscope, fully motorized
  • Nikon Perfect FocusTM focus stabilization system
  • High Numerical Aperture (NA) oil and water immersion objectives
  • Differential interference contrast microscopy
  • Okolab stage-top incubator with temperature and (CO2) control
  • Okolab objective heater
  • Motorized XYZ piezo stage
  • Two Andor Ultra EMCCD cameras
  • One Andor Neo sCMOS camera
  • Simultaneous two-color imaging (GFP/RFP or CFP/YFP)
  • Yokogawa CSU-W1 fully motorized confocal scanner unit
  • Efficient bypass mode allows for wide-field imaging
  • Six laser lines: 405nm, 445nm, 488nm, 515nm, 561nm, 640nm
  • FRAPPA laser-galvo scanner
  • FRAP, FRET, photoactivation and photoconversion applications
  • MetaMorphTM Premier software for image acquisition and analysis

Click here for information about objectives and lasers.

Click here for a detailed description. 

Leica Confocal

Leica TCS SL Point-Scanning Confocal

The Leica TCS SL is the Imaging Core’s point-scanning laser confocal system, ideal for imaging of fixed specimens.

System features:

  • Leica DM IRE2 inverted microscope stand
  • High Numerical Aperture (NA) oil and water immersion objectives
  • Differential interference contrast microscopy
  • Bioptechs heated chamber for live imaging
  • Leica objective heater for live imaging 
  • Z-galvo stage for fast acquisition of Z-stacks
  • Six laser lines: 458nm, 476nm, 488nm, 514nm, 543nm and 633nm 
  • Acousto-optical tunable filter (AOTF) for fast control of laser power
  • Ability to scan user-defined regions of interest
  • Three spectral 12-bit PMT detectors for three-channel fluorescence
  • One 12-bit PMT detector for transmitted light imaging
  • FRAP and FRET capability

Click here for information about objectives and lasers.

Click here for a detailed description.

Zeiss Axiophot

Zeiss Axiophot Microscope

The Zeiss Axiophot is a basic upright wide-field microscope for monochrome and true-color brightfield and epifluorescent imaging. 

Click here for information about objectives and filter cubes.

Click here for a detailed description. 


Training and Scheduling

Microscope Training

You must attend a training seminar to use the microscope. Seminars are held in Rightmire Hall. Please contact the Imaging Core manager, Paula Monsma, at 614-293-0939 or for the seminar schedule.

Andor Revolution WD Manuals and Handouts

Andor Training Handout: Downloading a copy of the handout does not override the need to attend a training seminar.

Andor Microscope Manual: This is a copy of the manual located in the confocal room.

Leica TCS SL Manuals and Handouts

Leica Training Handout: This is a copy of the handout, for your convenience. However, you will still need to attend a training seminar.

Leica Confocal Manual: This is a copy of the manual located in the confocal room.

Leica Microscope Manual: This is a copy of the manual located in the confocal room.


Microscope Scheduling

Scheduling for each instrument is coordinated online. Please contact the Facility Manager, Paula Monsma, at 614-293-0939 or for access to the scheduling calendar.

Access Policy

Trainee: Supervised, Monday – Friday, 9 a.m. – 5 p.m. 

  • Required experience: Seminar and minimum of two hands-on training sessions. Advancement decided on a case-by-case basis.

Standard User: Unsupervised, Monday – Friday, 9 a.m. – 5 p.m.

  • Required experience: Seminar and minimum of 10 unsupervised sessions. Advancement decided on a case-by-case basis.

Experienced User: Unsupervised, Monday – Friday, 9 a.m. – 5 p.m., as well as evenings and weekends

  • Required experience: Advancement decided on a case-by-case basis.


Technical Support

Your first contact for technical support should always be the Facility Manager, Paula Monsma.

Leica TCS SL

Leica OneCall: 866-830-0735

Technical Bulletins
Leica TCS SL






SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.

McGovern Vl, Iyer C, Arnold WD, Gombash SE, Blatnik AJ, Zaworski PG, Foust KD, Burghes AH.

Hum Mol Genet. 2015 Oct 1;24(19):5524-41. doi: 10.1093/hmg/ddv283. Epub 2015 Jul 23.

PMID: 26206889


Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.

Iyer C. McGovern VL, Murray JD, Gombash SE, Foust KD, Janssen PM, Burghes AH.

Hum Mol Genet. 2015 Nov 1;24(21):6160-73. doi: 10.1093/hmg/ddv332. Epub 2015 Aug 13.

PMID: 26276812


Dynamics of the slowing segmentation clock reveal alternating two-segment periodicity.

Shih NP, François P, Delaune EA, Amacher SL.

Development. 2015 May 15;142(10):1785-93. doi: 10.1242/dev.119057.

PMID: 25968314

Live-cell imaging of neurofilament transport in cultured neurons.

Uchida, A, Monsma, PC, Fenn, JD, Brown, A.

Methods Cell Biol. 2016;131:21-90. doi: 10.1016/bs.mcb.2015.07.001. Epub 2015 Sep 2.

PMID: 26794508


EphrinB2 controls vessel pruning through STAT1-JNK3 signaling.

Salvucci, O., Ohnuki, H., Maric, D., Hou, X., Li, X., Yoon, S.O., Segarra, M., Eberhart, C.G., Acker-Palmer, A., and Tosato, G.

Nat Commun. 2015 Mar 26;6:6576. doi: 10.1038/ncomms7576.

PMID: 25807892


Motoneuron development influences dorsal root ganglia survival and Schwann cell development in a vertebrate model of spinal muscular atrophy.

Hao LT, Duy PQ, Jontes JD, Beattie CE.

Hum Mol Genet. 2015 Jan 15;24(2):346-60. doi: 10.1093/hmg/ddu447. Epub 2014 Sep 1.

PMID: 25180019


Alpha-COP binding to the survival motor neuron protein SMN is required for neuronal process outgrowth.

Li H, Custer SK, Gilson T, Hao LT, Beattie CE, Androphy EJ.

Hum Mol Genet. 2015 Jan 15;24(2):346-60. doi: 10.1093/hmg/ddu447. Epub 2014 Sep 1.

PMID: 25180019


Recruitment of beta-arrestin into Neuronal Cilia Modulates Somatostatin Receptor Subtype 3 Ciliary Localization.

Green, J. A., C. L. Schmid, E. Bley, P. C. Monsma, A. Brown, L. M. Bohn, and K. Mykytyn.

Mol Cell Biol. 2015 Oct 26;36(1):223-35. doi: 10.1128/MCB.00765-15. Print 2016 Jan 1.

PMID: 26503786


In vivo assessment of contractile strength distinguishes differential gene function in skeletal muscle of zebrafish larvae.

Martin BL, Gallagher TL, Rastogi N, Davis JP, Beattie CE, Amacher SL, Janssen PM.

J Appl Physiol (1985). 2015 Oct 1;119(7):799-806. doi: 10.1152/japplphysiol.00447.2015. Epub 2015 Aug 6.

PMID: 26251513


Standardized orthotopic xenografts in zebrafish reveal glioma cell line specific characteristics and tumor cell heterogeneity.

Welker, A. S., Jaros, B. D., Puduvalli, V., Kaur, B., and Beattie, C. E.

Dis Model Mech. 2016 Sep 1;9(9):1063-5. doi: 10.1242/dmm.027235.

PMID: 27604693


Ankyrin-G directly binds to kinesin-1 to transport voltage-gated Na+ channels into axons.

Barry J, Gu Y, Jukkola P, O’Neill B, Gu H, Mohler PJ, Thirtamara-Rajamani K, and Gu C.

Dev Cell. 2014 Jan 27;28(2):117-31. doi: 10.1016/j.devcel.2013.11.023. Epub 2014 Jan 9.

PMID: 24412576


Primary cilia enhance kisspeptin receptor signaling on gonadotropin-releasing hormone neurons.

Koemeter-Cox AI, Sherwood TW, Green JA, Steiner RA, Berbari NF, Yoder BK, Kauffman AS, Monsma PC, Brown A, Askwith CC and Mykytyn K.

Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10335-40. doi: 10.1073/pnas.1403286111. Epub 2014 Jun 30.

PMID: 24982149


Calcium binding is essential for plastin 3 function in Smn-deficient motoneurons

Lyon AN, Pineda RH, Hao le T, Kudryashova E, Kudryashov DS, and Beattie CE.

Hum Mol Genet. 2014 Apr 15;23(8):1990-2004. doi: 10.1093/hmg/ddt595. Epub 2013 Nov 23.

PMID: 24271012


P75 regulates Purkinje cell firing by modulating SK channel activity through Rac 1.

Tian, J.B., Tep, C., Sadisivan, S., Smeyne, R., Zhu, M.X., and Yoon, S.O.

J Biol Chem. 2014 Nov 7;289(45):31458-72. doi: 10.1074/jbc.M114.589937. Epub 2014 Sep 24.

PMID: 25253694


Severing and end-to-end annealing of neurofilaments in neurons.

Uchida A, Çolakoğlu G, Wang L, Monsma PC and Brown A.

Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):E2696-705. doi: 10.1073/pnas.1221835110. Epub 2013 Jul 2.

PMID: 23821747


Temporal requirement for SMN in motoneuron development.

Hao LT, Duy PQ, Jontes JD, Wolman M, Granato M and Beattie CE.

Hum Mol Genet. 2013 Jul 1;22(13):2612-25. doi: 10.1093/hmg/ddt110. Epub 2013 Mar 3.

PMID: 23459934


Small Molecule Suppressors of Drosophila Kinesin Deficiency Rescue Motor Axon Development in a Zebrafish Model of Spinal Muscular Atrophy.

Gassman A, Hao LT, Bhoite L, Bradford CL, Chien C-B, Beattie CE, Manfredi JP.

PLoS One. 2013 Sep 4;8(9):e74325. doi: 10.1371/journal.pone.0074325. eCollection 2013.

PMID: 24023935


Kv3 channel assembly, trafficking and activity are regulated by zinc through different binding sites.

Gu Y, Barry J, and Gu C.

J Physiol. 2013 May 15;591(10):2491-507. doi: 10.1113/jphysiol.2013.251983. Epub 2013 Feb 18.

PMID: 23420657


Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels.

Barry J, Xu M, Gu Y, Dangel A, Shrestha C, and Gu C.

J Cell Sci. 2013 May 1;126(Pt 9):2027-41. doi: 10.1242/jcs.122234. Epub 2013 Mar 13.

PMID: 23487040


Astrocytes differentially respond to inflammatory autoimmune insults and imbalances of neural activity.

Jukkola P, Guerrero T, Gray V, and Gu C.

Acta Neuropathol Commun. 2013 Oct 23;1:70. doi: 10.1186/2051-5960-1-70.

PMID: 24252623


Oral administration of a small molecule targeted to block proNGF binding to p75 promotes myelin sparing and functional recovery after spinal cord injury.

Tep C, Lim, TH, Ko PO, Getahun S, Goettl VM, Massa S, Basso M, Longo FM, and Yoon SO.

J Neurosci. 2013 Jan 9;33(2):397-410. doi: 10.1523/JNEUROSCI.0399-12.2013.

PMID: 23303920


Anti-tumor efficacy of 34.5ENVE: a transcriptionally retargeted and “Vstat120” oncolytic virus. 

Yoo JY, Haseley A, Bratasz A, Chiocca EA, Zhang JY, Cain D, Powell K, Kaur B.

Mol Ther. 2012 Feb;20(2):287-97. doi: 10.1038/mt.2011.208. Epub 2011 Oct 25.

PMID: 22031239


Alternative splicing regulates Kv3.1 polarized targeting to adjust the maximal spiking frequency.

Gu YZ, Barry J, McDougle R, Terman D, and Gu C.

J Biol Chem. 2012 Jan 13;287(3):1755-69. doi: 10.1074/jbc.M111.299305. Epub 2011 Nov 21.

PMID: 22105078


K+ channel alterations in the progression of experimental autoimmune encephalomyelitis.

Jukkola P, Lovett-Racke AE, Zamvil SS, and Gu C.

Neurobiol Dis. 2012 Aug;47(2):280-93. doi: 10.1016/j.nbd.2012.04.012. Epub 2012 Apr 24.

PMID: 22560931


Myelination of rodent hippocampal neurons in culture. 

Gardner A, Jukkola P, and Gu C.

Nat Protoc. 2012 Oct;7(10):1774-82. doi: 10.1038/nprot.2012.100. Epub 2012 Sep 6.

PMID: 22955693


Dual modification of Alzheimer's disease PHF-tau protein by lysine methylation and ubiquitylation: a mass spectrometry approach.

Thomas SN, Funk KE, Wan Y, Liao Z, Davies P, Kuret J, Yang AJ.

Acta Neuropathol. 2012 Jan;123(1):105-17. doi: 10.1007/s00401-011-0893-0. Epub 2011 Oct 28.

PMID: 22033876


Survival motor neuron affects plastin 3 protein levels leading to motor defects.

Hao le T, Wolman M, Granato M, Beattie CE.

J Neurosci. 2012 Apr 11;32(15):5074-84. doi: 10.1523/JNEUROSCI.5808-11.2012.

PMID: 22496553


JNK3 perpetuates metabolic stress induced by Abeta peptides.

Yoon SO, Park DJ, Ryu CH, Ozer HG, Tep C, Shin YJ, Lim TH, Pastorino L, Kunwar AJ, Walton JC, Nagahara AH, Lu KP, Nelson RJ, Tuszynski MH, Huang K.

Neuron. 2012 Sep 6;75(5):824-37. doi: 10.1016/j.neuron.2012.06.024.

PMID: 22958823


FluoroMyelin™ Red is a bright, photostable and non-toxic fluorescent stain for live imaging of myelin.

Monsma PC and Brown A.

J Neurosci Methods. 2012 Aug 15;209(2):344-50. doi: 10.1016/j.jneumeth.2012.06.015. Epub 2012 Jun 26.

PMID: 22743799


Neurofilaments are flexible polymers that often fold and unfold, but they move in a fully extended configuration.

Taylor NJ, Wang L and Brown A.

Cytoskeleton (Hoboken). 2012 Jul;69(7):535-44. doi: 10.1002/cm.21039. Epub 2012 Jun 12.

PMID: 22693112


A SMN-dependent U12 splicing event essential for motor circuit function.

Lotti F, Imlach WL, Saieva L, Beck ES, Hao LT, Li DK, Jiao W, Mentis GZ, Beattie CE, McCabe, BD and Pellizzoni L.

Cell. 2012 Oct 12;151(2):440-54. doi: 10.1016/j.cell.2012.09.012.

PMID: 23063131


Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN.

Foust KD, Wang X, McGovern VL, Braun L, Bevan AK, Haidet AM, Le TT, Morales PR, Rich MM, Burghes AH, Kaspar BK.

Nat Biotechnol. 2010 Mar;28(3):271-4. doi: 10.1038/nbt.1610. Epub 2010 Feb 28.

PMID: 20190738


A genetic model of amyotrophic lateral sclerosis in zebrafish displays phenotypic hallmarks of motoneuron disease.

Ramesh T, Lyon AN, Pineda RH, Wang C, Janssen PM, Canan BD, Burghes AH, Beattie CE.

Dis Model Mech. 2010 Sep-Oct;3(9-10):652-62. doi: 10.1242/dmm.005538. Epub 2010 May 26.

PMID: 20504969


Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments.

Uchida A, Alami NH, Brown A.

Mol Biol Cell. 2009 Dec;20(23):4997-5006. doi: 10.1091/mbc.E09-04-0304. Epub 2009 Oct 7.

PMID: 19812246


Intermediate filaments exchange subunits along their length and elongate by end-to-end annealing.

Colakoğlu G, Brown A.

J Cell Biol. 2009 Jun 1;185(5):769-77. doi: 10.1083/jcb.200809166. Epub 2009 May 25.

PMID: 19468066


Myosin Va increases the efficiency of neurofilament transport by decreasing the duration of long-term pauses.

Alami NH, Jung P, Brown A.

J Neurosci. 2009 May 20;29(20):6625-34. doi: 10.1523/JNEUROSCI.3829-08.2009.

PMID: 19458233


Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects.

Boon KL, Xiao S, McWhorter ML, Donn T, Wolf-Saxon E, Bohnsack MT, Moens CB, Beattie CE.

Hum Mol Genet. 2009 Oct 1;18(19):3615-25. doi: 10.1093/hmg/ddp310. Epub 2009 Jul 10.

PMID: 19592581

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