Our Injury Core supports innovative neurological research here at Ohio State.

CNS/PNS Nerve Injury Core

The CNS (central nervous system)/PNS (peripheral nervous system) Injury Core provides technical support to generate animal models of injury and to characterize their behavioral impairment after injury. 

The services available in this Central Nervous System/Peripheral Nervous System Core provide unique and valuable services to investigators funded by the National Institute of Neurological Disorders and Stroke (NINDS). These services provide an array of surgical and behavioral techniques that are not typically located together in a single laboratory.

This core provides standardized peripheral nerve injuries, spinal contusion injuries and a variety of intraspinal or intracranial microlesions induced by microinjection of specific toxins or demyelinating agents. This core also provides behavioral tools commonly used in spinal cord injury (SCI) studies, which differ from those offered in the Behavioral Core. These core services provide an opportunity for investigators wishing to test the response of transgenic animals to CNS/PNS injury or to test the efficacy of therapeutic agents.

The primary service of this core is induced standardized peripheral nerve injuries or spinal cord injuries in small animals. The core also provides behavioral tools commonly used in spinal cord injury studies. Although this support covers costs of the behavioral technician, it does not cover equipment or animal per diem charges.

Services available: 
  • Sciatic nerve transection
  • Spinal contusion injury
  • Focal spinal injury via microinjection of specific toxins (e.g., LPS and zymosan)
  • Focal spinal demyelination via microinjection of lysolecithin or ethidium bromide

Please see our Terms of Service and User Fee Structure




Ankeny DP, Popovich PG. Mechanisms and implications of adaptive immune responses after traumatic spinal cord injury. Neuroscience. 2009 Feb 6;158(3):1112-21. doi: 10.1016/j.neuroscience.2008.07.001. Epub 2008 Jul 4. Review. PMCID: PMC2661571

Hoschouer EL, Yin FQ, Jakeman LB. L1 cell adhesion molecule is essential for the maintenance of hyperalgesia after spinal cord injury. Exp Neurol. 2009 Mar;216(1):22-34.doi:10.1016/j.expneurol.2008.10.025. Epub 2008 Nov 13. PMCID: PMC3691996

Schonberg DL, McTigue DM. Iron is essential for oligodendrocyte genesis following intraspinal macrophage activation. Exp Neurol. 2009 Jul;218(1):64-74. doi: 10.1016/j.expneurol. 2009.04.005. Epub 2009 Apr 15. PMCID: PMC2703190

Neigh GN, Karelina K, Glasper ER, Bowers SL, Zhang N, Popovich PG, DeVries AC. Anxiety after cardiac arrest/cardiopulmonary resuscitation: exacerbated by stress and prevented by minocycline. Stroke. 2009 Nov;40(11):3601-7. doi: 10.1161/STROKEAHA.109.564146. Epub 2009 Sep 17. PMCID: PMC2805193
Ankeny DP, Guan Z, Popovich PG. B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice. J Clin Invest. 2009 Oct;119(10):2990-9. doi: 10.1172/JCI39780. Epub 2009 Sep 21. PMCID: PMC2752085

Naphade SB, Kigerl KA, Jakeman LB, Kostyk SK, Popovich PG, Kuret J. Progranulin expression is upregulated after spinal contusion in mice. Acta Neuropathol. 2010 Jan;119(1):123-33. Epub 2009 Nov 28. NIHMSID: NIHMS646937 

Hoschouer EL, Basso DM, Jakeman LB. Aberrant sensory responses are dependent on lesion severity after spinal cord contusion injury in mice. Pain. 2010 Feb;148(2):328-42. Epub 2009 Dec 22. PMCID: PMC2815266 

Almad A, McTigue DM. Chronic expression of PPAR-delta by oligodendrocyte lineage cells in the injured rat spinal cord.J Comp Neurol. 2010 Mar 15;518(6):785-99. PMCID: PMC4026285

Detloff MR, Clark LM, Hutchinson KJ, Kloos AD, Fisher LC, Basso DM. Validity of acute and chronic tactile sensory testing after spinal cord injury in rats. Exp Neurol. 2010 Oct;225(2):366-76. Epub 2010 Jul 17. NIHMSID: NIHMS224236

Awad H, Ankeny DP, Guan Z, Wei P, McTigue DM, Popovich PG. A mouse model of ischemic spinal cord injury with delayed paralysis caused by aortic cross-clamping. Anesthesiology. 2010 Oct;113(4):880-91. PMCID: PMC2945453

Jakeman LB, Hoschouer EL, Basso DM. Injured mice at the gym: review, results and considerations for combining chondroitinase and locomotor exercise to enhance recovery after spinal cord injury. Brain Res Bull. 2011 Mar 10;84(4-5):317-26. Epub 2010 Jun 15. PMCID: PMC3030989

White RE, Rao M, Gensel JC, McTigue DM, Kaspar BK, Jakeman LB. Transforming growth factor α transforms astrocytes to a growth-supportive phenotype after spinal cord injury. J Neurosci. 2011 Oct 19;31(42):15173-87. doi: 10.1523/JNEUROSCI.3441-11.2011. PubMed PMID: 22016551; PubMed Central PMCID: PMC3213757.

Donnelly DJ, Longbrake EE, Shawler TM, Kigerl KA, Lai W, Tovar CA, Ransohoff RM, Popovich PG. Deficient CX3CR1 signaling promotes recovery after mouse spinal cord injury by limiting the recruitment and activation of Ly6Clo/iNOS+ macrophages. J Neurosci. 2011 Jul 6;31(27):9910-22. PMCID: PMC3139517

Alexander JK, Cox GM, Tian JB, Zha AM, Wei P, Kigerl KA, Reddy MK, Dagia NM, Sielecki T, Zhu MX, Satoskar AR, McTigue DM, Whitacre CC, Popovich PG. Macrophage migration inhibitory factor (MIF) is essential for inflammatory and neuropathic pain and enhances pain in response to stress. Exp Neurol. 2012 Aug;236(2):351-62. doi: 10.1016/j.expneurol.2012.04.018. Epub 2012 May 1. PubMed PMID: 22575600; PubMed Central PMCID: PMC3392533.

Schonberg DL, Goldstein EZ, Sahinkaya FR, Wei P, Popovich PG, McTigue DM. Ferritin stimulates oligodendrocyte genesis in the adult spinal cord and can be transferred from macrophages to NG2 cells in vivo. J Neurosci, 32(16): 5374-5384; 2012. PMCID: PMC3521599

Kigerl KA, Ankeny DP, Garg SK, Wei P, Guan Z, Lai W, McTigue DM, Banerjee R, Popovich PG. System xc- regulates microglia and macrophage glutamate excitotoxicity in vivo. Exp Neurol 233: 333-341; 2012. PMCID: PMC3268895

Yoon S, Park D, Ryu J, Ozer H, Tep C, Shin Y, Lim TH, Pastorino L, Kunwar AJ, Walton JC, Nagahara AH, Lu KP, Nelson RJ, Tuszynski MH, Huang K. 2012. JNK3 perpetuates Metabolic Stress Induced by Aβ Peptides. Neuron 75:824-37.PMCID: PMC3438522

Tep C, Lim TH, Ko PO, Getahun S, Ryu JC, Goettl VM, Massa SM, Basso M, Longo FM, Yoon SO. Oral administration of a small molecule targeted to block proNGF binding to p75 promotes myelin sparing and functional recovery after spinal cord injury. J Neurosci. 2013 Jan 9;33(2):397-410. doi: 10.1523/JNEUROSCI.0399-12.2013. Erratum in: J Neurosci. 2014 Jan 29;34(5):2012. PubMed PMID: 23303920; PubMed Central PMCID: PMC3710149.

Cox GM, Kithcart AP, Pitt D, Guan Z, Alexander J, Williams JL, Shawler T, Dagia NM, Popovich PG, Satoskar AR, Whitacre CC. Macrophage migration inhibitory factor potentiates autoimmune-mediated neuroinflammation. J Immunol. 2013 Aug 1;191(3):1043-54. doi: 10.4049/jimmunol.1200485. Epub 2013 Jun 24. NIHMSID: NIHMS485628. PMID:23797673

Tu TW, Kim JH, Yin FQ, Jakeman LB, Song SK. The impact of myelination on axon sparing and locomotor function recovery in spinal cord injury assessed using diffusion tensor imaging. NMR Biomed. 2013 Jun 18. doi: 10.1002/nbm.2981. PMCID: PMC3800477

Wang Y, Nowicki MO, Wang X, Arnold WD, Fernandez SA, Mo X, Wechuk J, Krisky D, Goss J, Wolfe D, Popovich PG, Lawler S, Chiocca EA. Comparative effectiveness of antinociceptive gene therapies in animal models of diabetic neuropathic pain. Gene Ther. 2013 Jul;20(7):742-50. doi: 10.1038/gt.2012.90. Epub 2012 Dec 13. NIHMSID: NIHMS646944 PMID: 23235561

Zhang Y, Guan Z, Reader B, Shawler T, Mandrekar-Colucci S, Huang K, Weil Z, Bratasz A, Wells J, Powell ND, Sheridan JF, Whitacre CC, Rabchevsky AG, Nash MS, Popovich PG. Autonomic Dysreflexia Causes Chronic Immune Suppression after Spinal Cord Injury J Neurosci. 2013 Aug 7;33(32):12970-12981. PMCID: PMC3735880 
Sauerbeck A, Schonberg DL, Law L, McTigue DM. Systemic iron chelation results in limited functional recovery and histological recovery after traumatic spinal cord injury in rats. Exp Neurol, 248: 53-61; 2013 NIHMSID: NIHMS646945

Hansen CN, Fisher LC, Deibert RJ, Jakeman LB, Zhang H, Noble-Haeusslein L, White S, Basso DM. Elevated mmp-9 in the lumbar cord early after thoracic spinal cord injury impedes motor relearning in mice. J Neurosci. 2013 Aug 7;33(32):13101-11. doi: 10.1523/JNEUROSCI.1576-13. 2013. PMCID: PMC3735886

Leiblein-Boff JC, Shea DT, Wei P, Deng Z, Quan N, Bilbo SB, Bailey MT, McTigue DM, and Godbout JP (2013). Neonatal E.Coli infection causes neuro-behavioral deficits associated with hypomyelination and neuronal sequestration of iron. J Neurosci, 33(41): 16334 – 45. PMCID: PMC3792468

Nielson JL, Guandique CF, Liu AW, Burke DA, Lash AT, Moseanko R, Hawbecker S, Strand SC, Zdunowski S, Irvine KA, Brock JH, Nout-Lomas YS, Gensel JC, Anderson KD, Segal MR, Rosenzweig ES, Magnuson DS, Whittemore SR, McTigue DM, Popovich PG, Rabchevsky AG, Scheff SW, Steward O, Courtine G, Edgerton VR, Tuszynski MH, Beattie MS, Bresnahan JC, Ferguson AR. Development of a database for translational spinal cord injury research. J Neurotrauma. 2014 Nov 1;31(21):1789-99. doi: 10.1089/neu.2014.3399. Epub 2014 Jul 31. PubMed PMID: 25077610; PubMed Central PMCID: PMC4186058.

Sahinkaya FR, Milich L, McTigue DM. (2014) Changes in NG2 cells and oligodendrocytes in a new model of intraspinal hemorrhage. Exp Neurol, 255C: 113-126. PMCID: PMC4036698

Fenn AM, Hall JC, Gensel JC, Popovich PG, and Godbout JP (2014). IL-4 signaling drives a unique Arginase+/IL-1b+ microglia phenotype and recruits macrophages to the inflammatory CNS: consequences of age-related deficits in IL-4Ra after traumatic spinal cord injury. J Neurosci 34: 8904-17. PMCID: PMC4069360

Fenn AM, Skendelas JP, Moussa DN, Muccigrosso MM, Popovich PG, Lifshitz J, Eiferman DS, Godbout JP. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38. doi: 10.1089/neu.2014.3514. Epub 2014 Nov 13. PubMed PMID: 25070744; PubMed Central PMCID: PMC4291210. 

Sauerbeck AD, Laws JL, Bandaru VV, Popovich PG, Haughey NJ, McTigue DM. Spinal cord injury causes chronic liver pathology in rats. J Neurotrauma. 2015 Feb 1;32(3):159-69. doi: 10.1089/neu.2014.3497. Epub 2014 Oct 21. PubMed PMID: 25036371; PubMed Central PMCID: PMC4298754.

Popovich PG, Tovar CA, Lemeshow S, Yin Q, Jakeman LB. Independent evaluation of the anatomical and behavioral effects of Taxol in rat models of spinal cord injury. Exp Neurol. 2014 Nov;261:97-108. doi: 10.1016/j.expneurol.2014.06.020. Epub 2014 Jul 3. PubMed PMID: 24999028; PubMed Central PMCID: PMC4194241.
Fenn AM, Gensel JC, Huang Y, Popovich PG, Lifshitz J, and Godbout JP (2014). Immune activation promotes depression 1 month after diffuse brain injury: a role for primed microglia. Biol Psychiatry 76(7): 575-84. PMCID: PMC4000292

Sauerbeck AD, Laws JL, Bandaru VV, Popovich PG, Haughey NJ, McTigue DM. Spinal cord injury causes chronic liver pathology in rats. J Neurotrauma. 2015 Feb 1;32(3):159-69. doi: 10.1089/neu.2014.3497. Epub 2014 Oct 21. PubMed PMID: 25036371; PubMed Central PMCID: PMC4298754.

Hesp ZC, Goldstein EZ, Miranda CJ, Kaspar BK, McTigue DM. Chronic oligodendrogenesis and remyelination after spinal cord injury in mice and rats. J Neurosci. 2015 Jan 21;35(3):1274-90. doi: 10.1523/JNEUROSCI.2568-14.2015. Erratum in: J Neurosci. 2015 May 6;35(18):7313. Goldstein, Evan A [Corrected to Goldstein, Evan Z] and Kaspar, Brain K [Corrected to Kaspar, Brian K]. PubMed PMID: 25609641; PubMed Central PMCID: PMC4300327.

Puga DA, Tovar CA, Guan Z, Gensel JC, Lyman MS, McTigue DM, Popovich PG. Stress exacerbates neuron loss and microglia proliferation in a rat model of excitotoxic lower motor neuron injury. Brain Behav Immun. 2015 Oct;49:246-54. doi: 10.1016/j.bbi.2015.06.006. Epub 2015 Jun 19. PubMed PMID: 26100488; PubMed Central PMCID: PMC4567453.

Gensel JC, Wang Y, Guan Z, Beckwith KA, Braun KJ, Wei P, McTigue DM, Popovich PG. Toll-Like Receptors and Dectin-1, a C-Type Lectin Receptor, Trigger Divergent Functions in CNS Macrophages. J Neurosci. 2015 Jul 8;35(27):9966-76. doi: 10.1523/JNEUROSCI.0337-15.2015. PubMed PMID: 26156997; PubMed Central PMCID: PMC4495245.

Carpenter RS, Kigerl KA, Marbourg JM, Gaudet AD, Huey D, Niewiesk S, Popovich PG. Traumatic spinal cord injury in mice with human immune systems. Exp Neurol. 2015 Sep;271:432-44. doi: 10.1016/j.expneurol.2015.07.011. Epub 2015 Jul 17. PubMed PMID: 26193167; PubMed Central PMCID: PMC4586484.

Contact the Department of Neuroscience

The Ohio State University Wexner Medical Center
Department of Neuroscience

333 W. 10th Ave. Columbus, OH 43210

Melissa Stenger

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