Our Physiology Core focuses on electrophysiology and muscle physiology

This Core expands the capabilities of neuroscience research on campus by making available specialized expertise, equipment and assistance to support electrophysiological analyses of signal transduction and synaptic function in cultured cells, tissue slices and zebrafish embryos.

The Electrophysiology Core is located in 4118 Graves Hall.

Microelectrodes record intracellular activity of individual neurons in rat hypothalamic tissue slices. 

Electrode patched on neuron
Recorded neuron filled with fluorescent dye
Recorded action potentials
Microelectrode in a brain slice

Available services:

  • Technical expertise, equipment and consultation for conducting electrophysiological studies
  • Extracellular or patch clamp recordings to screen for alterations in synaptic transmission, such as measurement of EPSP or EPSC or alteration in LTP or LTD, in cerebellar, hippocampal, hypothalamic and spinal cord slices
  • Electrophysiological studies of voltage- and ligand-channels in isolated or cultured neurons

Please see our Terms of Service for specifics regarding use of this core.

Publications

Iscru E, Serinagaoglu Y, Schilling K, Tian J, Bowers-Kidder SL, Zhang R, Morgan JI, DeVries AC, Nelson RJ, Zhu MX, Oberdick J. Sensorimotor enhancement in mouse mutants lacking the Purkinje cell-specific Gi/o modulator, Pcp2(L7). Mol Cell Neurosci. 2009 Jan;40(1):62-75. doi: 10.1016/j.mcn.2008.09.002. Epub 2008 Oct 1. PMCID: PMC2637474

Hu H, Bandell M, Petrus MJ, Zhu MX, Patapoutian A. Zinc activates damage-sensing TRPA1 ion channels. Nat Chem Biol. 2009 Mar;5(3):183-90. doi: 10.1038/nchembio.146. Epub 2009 Feb 8. PMCID: PMC2677965

Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, Tang J, Rietdorf K, Teboul L, Chuang KT, Lin P, Xiao R, Wang C, Zhu Y, Lin Y, Wyatt CN, Parrington J, Ma J, Evans AM, Galione A, Zhu MX. NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature. 2009 May 28;459(7246):596-600. doi: 10.1038/nature08030. Epub 2009 Apr 22. PMCID: PMC2761823

Tsvilovskyy VV, Zholos AV, Aberle T, Philipp SE, Dietrich A, Zhu MX, Birnbaumer L, Freichel M, Flockerzi V. Deletion of TRPC4 and TRPC6 in mice impairs smooth muscle contraction and intestinal motility in vivo. Gastroenterology. 2009 Oct;137(4):1415-24. doi: 10.1053/j.gastro.2009.06.046. Epub 2009 Jun 21. PMCID: PMC2757464

Gross SA, Guzmán GA, Wissenbach U, Philipp SE, Zhu MX, Bruns D, Cavalié A. TRPC5 is a Ca2+-activated channel functionally coupled to Ca2+-selective ion channels. J Biol Chem. 2009 Dec 4;284(49):34423-32. doi: 10.1074/jbc.M109.018192. Epub 2009 Oct 8. PMCID: PMC2797210

Foust KD, Wang X, McGovern VL, Braun L, Bevan AK, Haidet AM, Le TT, Morales PR, Rich MM, Burghes AH, Kaspar BK. Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN Nat Biotechnol. 2010 Mar;28(3):271-4. Epub 2010 Feb 28. PMCID: PMC2889698

Hu H, Tian J, Zhu Y, Wang C, Xiao R, Herz JM, Wood JD, Zhu MX. Activation of TRPA1 channels by fenamate nonsteroidal anti-inflammatory drugs. Pflugers Arch. 2010 Mar;459(4):579-92. Epub 2009 Nov 4. PMCID: PMC2828537

Zhu MX, Ma J, Parrington J, Galione A, Evans AM. TPCs: Endolysosomal channels for Ca2+ mobilization from acidic organelles triggered by NAADP. FEBS Lett. 2010 May 17;584(10):1966-74. Epub 2010 Feb 14. Review. PMCID: PMC2867333

Colton CK, Kong Q, Lai L, Zhu MX, Seyb KI, Cuny GD, Xian J, Glicksman MA, Lin CL. Identification of translational activators of glial glutamate transporter EAAT2 through cell-based high-throughput screening: an approach to prevent excitotoxicity. J Biomol Screen. 2010 Jul;15(6):653-62. Epub 2010 May 27. PMCID: PMC3016154

Sherwood TW, Lee KG, Gormley MG, Askwith CC. Heteromeric acid-sensing ion channels (ASICs) composed of ASIC2b and ASIC1a display novel channel properties and contribute to acidosis-induced neuronal death. J Neurosci. 2011 Jun 29;31(26):9723-34. PMCID: PMC3160670

Hester ME, Murtha MJ, Song S, Rao M, Miranda CJ, Meyer K, Tian J, Boulting G, Schaffer DV, Zhu MX, Pfaff SL, Gage FH, Kaspar BK. Rapid and efficient generation of functional motor neurons from human pluripotent stem cells using gene delivered transcription factor codes. Mol Ther. 2011 Oct;19(10):1905-12. doi: 10.1038/mt.2011.135. Epub 2011 Jul 19. PMCID: PMC3188742

Alexander JK, Cox GM, Tian JB, Zha AM, Wei P, Kigerl KA, Reddy MK, Dagia NM, Sielecki T, Zhu MX, Satoskar AR, McTigue DM, Whitacre CC, Popovich PG. Macrophage migration inhibitory factor (MIF) is essential for inflammatory and neuropathic pain and enhances pain in response to stress. Exp Neurol. 2012 Aug;236(2):351-62. doi: 10.1016/j.expneurol.2012.04.018. Epub 2012 May 1. PubMed PMID: 22575600; PubMed Central PMCID: PMC3392533.

Sherwood TW, Frey EN, Askwith CC. Structure and activity of the acid-sensing ion channels. Am. J. Physiol. 2012 Oct 1;303(7):C699-710. Epub 2012 Jul 25. PMCID: PMC3469599

Tep C, Lim TH, Ko PO, Getahun S, Ryu JC, Goettl VM, Massa SM, Basso M, Longo FM, Yoon SO. Oral administration of a small molecule targeted to block proNGF binding to p75 promotes myelin sparing and functional recovery after spinal cord injury. J Neurosci. 2013 Jan 9;33(2):397-410. doi: 10.1523/JNEUROSCI.0399-12.2013. Erratum in: J Neurosci. 2014 Jan 29;34(5):2012. PubMed PMID: 23303920; PubMed Central PMCID: PMC3710149.

Tian J, Tep C, Zhu MX, Yoon SO. Changes in Spontaneous firing patterns of cerebellar Purkinje cells in p75 knockout mice. Cerebellum. 2013 Jun;12(3):300-3. doi: 10.1007/s12311-012-0439-6. PubMed PMID: 23307658; PubMed Central PMCID: PMC3643992.

Frey EN, Pavlovicz RE, Wegman CJ, Li C, Askwith CC. Conformational changes in the lower palm domain of ASIC1a contribute to desensitization and RFamide modulation. PLoS One. 2013 Aug 14;8(8):e71733. PMCID: PMC3743763

Koemeter-Cox AI, Sherwood TW, Green JA, Steiner RA, Berbari NF, Yoder BK, Kauffman AS, Monsma PC, Brown A, Askwith CC, and Mykytyn K. Primary cilia enhance kisspeptin receptor signaling on gonadotropin-releasing hormone neurons. Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10335-40. PMCID: PMC4104922

Tian J, Tep C, Benedick A, Saidi N, Ryu JC, Kim ML, Sadasivan S, Oberdick J, Smeyne R, Zhu MX, Yoon SO. p75 regulates Purkinje cell firing by modulating SK channel activity through Rac1. J Biol Chem. 2014 Nov 7;289(45):31458-72. Epub 2014 Sep 24. PMCID: PMC4223344

Meyer K, Ferraiuolo L, Miranda CJ, Likhite S, McElroy S, Renusch S, Ditsworth D, Lagier-Tourenne C, Smith RA, Ravits J, Burghes AH, Shaw PJ, Cleveland DW, Kolb SJ, Kaspar BK. Direct conversion of patient fibroblasts demonstrates non-cell autonomous toxicity of astrocytes to motor neurons in familial and sporadic ALS. Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):829-32. Epub 2013 Dec 30. PMCID: PMC3896192

Vick JS, Askwith CC. ASICs and neuropeptides. Neuropharmacology. 2015 Jul;94:36-41. Epub 2015 Jan 12. PMCID: PMC4458394

Boxwell, A.J., Chen, Z., Mathes, C.M., Spector, A.C., Le Roux, C.W., Travers, S.P., Travers, J.B. Effects of high- fat diet and gastric bypass on neurons in the caudal solitary nucleus. Physiology and Behavior, Physiol Behav. 2015 Dec 1;152(Pt B):329-39. Epub 2015 Jul 26. PMCID: PMC4661085

Green JA, Schmid CL, Bley E, Monsma PC, Brown A, Bohn LM, Mykytyn K. Recruitment of β-arrestin into Neuronal Cilia Modulates Somatostatin Receptor Subtype 3 Ciliary Localization. Mol Cell Biol. 2015 Oct 26. pii: MCB.00765-15. Epub ahead of print. PMID: 26503786

Gallego-Perez D, Otero JJ, Czeisler C, Ma J, Ortiz C, Gygli P, Catacutan FP, Gokozan HN, Cowgill A, Sherwood TW, Ghatak S, Malkoc V, Zhao X, Wang X, Adler A, Leong L, Wulff B, Wilgus TA, Askwith CC, Khanna S, Rink C, Sen C, Lee LJ* Deterministic Non-viral Transfection Leads to Superior Reprogramming and Uncovers Novel Stochastic Barriers to Induced Neuron Formation. Nanomedicine, NMB In Press.

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