Core Services

This Core expands the capabilities of neuroscience research on campus by making available specialized expertise, equipment and assistance to support electrophysiological analyses of signal transduction and synaptic function in cultured cells, tissue slices and zebrafish embryos.


Available services:

  • Technical expertise, equipment and consultation for conducting electrophysiological studies
  • Extracellular or patch clamp recordings to screen for alterations in synaptic transmission, such as measurement of EPSP or EPSC or alteration in LTP or LTD, in cerebellar, hippocampal, hypothalamic and spinal cord slices
  • Electrophysiological studies of voltage- and ligand-channels in isolated or cultured neurons

Please see our Terms of Service and User Fee Structure for specifics regarding use of this core.

Publications

Publications

2018

Dissipation of transmembrane potassium gradient is the main cause of cerebral ischemia-induced depolarization in astrocytes and neurons.
Du Y, Wang W, Lutton AD, Kiyoshi CM, Ma B, Taylor AT, Olesik JW, McTigue DM, Askwith CC, Zhou M. 
Exp Neurol. 2018 May;303:1-11. doi: 10.1016/j.expneurol.2018.01.019. Epub 2018 Feb 3. PMID: 29407729

Pyridazine-derivatives Enhance Structural and Functional Plasticity of Tripartite Synapse Via Activation of Local Translation in Astrocytic Processes.
Foster JB, Zhao F, Wang X, Xu Z, Lin K, Askwith CC, Hodgetts KJ, Lin CG. 
Neuroscience. 2018 Sep 15;388:224-238. doi: 10.1016/j.neuroscience.2018.07.028. Epub 2018 Jul 27.
PMID: 30056115

Syncytial isopotentiality: A system-wide electrical feature of astrocytic networks in the brain.
Kiyoshi CM, Du Y, Zhong S, Wang W, Taylor AT, Xiong B, Ma B, Terman D, Zhou M.
Glia. 2018 Dec;66(12):2756-2769. doi: 10.1002/glia.23525. Epub 2018 Sep 12. 
PMID: 30277621.

2016

2015

ASICs and neuropeptides.

Vick JS, Askwith CC.

Neuropharmacology. 2015 Jul;94:36-41. doi: 10.1016/j.neuropharm.2014.12.012. Epub 2015 Jan 12.

PMID: 25592215

 

Effects of high- fat diet and gastric bypass on neurons in the caudal solitary nucleus.

Boxwell, A.J., Chen, Z., Mathes, C.M., Spector, A.C., Le Roux, C.W., Travers, S.P., Travers, J.B.

Physiol Behav. 2015 Dec 1;152(Pt B):329-39. doi: 10.1016/j.physbeh.2015.07.025. Epub 2015 Jul 26.

PMID: 26216080

 

Recruitment of β-arrestin into Neuronal Cilia Modulates Somatostatin Receptor Subtype 3 Ciliary Localization.

Green JA, Schmid CL, Bley E, Monsma PC, Brown A, Bohn LM, Mykytyn K.

Mol Cell Biol. 2015 Oct 26;36(1):223-35. doi: 10.1128/MCB.00765-15. Print 2016 Jan 1.

PMID: 26503786

 

Deterministic Non-viral Transfection Leads to Superior Reprogramming and Uncovers Novel Stochastic Barriers to Induced Neuron Formation.

Gallego-Perez D, Otero JJ, Czeisler C, Ma J, Ortiz C, Gygli P, Catacutan FP, Gokozan HN, Cowgill A, Sherwood TW, Ghatak S, Malkoc V, Zhao X, Wang X, Adler A, Leong L, Wulff B, Wilgus TA, Askwith CC, Khanna S, Rink C, Sen C, Lee LJ*

Nanomedicine. 2016 Feb;12(2):399-409. doi: 10.1016/j.nano.2015.11.015. Epub 2015 Dec 19.

PMID: 26711960

2014

Conformational changes in the lower palm domain of ASIC1a contribute to desensitization and RFamide modulation.

Frey EN, Pavlovicz RE, Wegman CJ, Li C, Askwith CC.

PLoS One. 2013 Aug 14;8(8):e71733. doi: 10.1371/journal.pone.0071733. eCollection 2013.

PMID: 23977127

 

Primary cilia enhance kisspeptin receptor signaling on gonadotropin-releasing hormone neurons.

Koemeter-Cox AI, Sherwood TW, Green JA, Steiner RA, Berbari NF, Yoder BK, Kauffman AS, Monsma PC, Brown A, Askwith CC, and Mykytyn K.

Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10335-40. doi: 10.1073/pnas.1403286111. Epub 2014 Jun 30.

PMID: 24982149

 

p75 regulates Purkinje cell firing by modulating SK channel activity through Rac1.

Tian J, Tep C, Benedick A, Saidi N, Ryu JC, Kim ML, Sadasivan S, Oberdick J, Smeyne R, Zhu MX, Yoon SO.

J Biol Chem. 2014 Nov 7;289(45):31458-72. doi: 10.1074/jbc.M114.589937. Epub 2014 Sep 24.

PMID: 25253694

 

Direct conversion of patient fibroblasts demonstrates non-cell autonomous toxicity of astrocytes to motor neurons in familial and sporadic ALS.

Meyer K, Ferraiuolo L, Miranda CJ, Likhite S, McElroy S, Renusch S, Ditsworth D, Lagier-Tourenne C, Smith RA, Ravits J, Burghes AH, Shaw PJ, Cleveland DW, Kolb SJ, Kaspar BK.

Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):829-32. doi: 10.1073/pnas.1314085111. Epub 2013 Dec 30.

PMID: 24379375

2013

Oral administration of a small molecule targeted to block proNGF binding to p75 promotes myelin sparing and functional recovery after spinal cord injury.

Tep C, Lim TH, Ko PO, Getahun S, Ryu JC, Goettl VM, Massa SM, Basso M, Longo FM, Yoon SO.

J Neurosci. 2013 Jan 9;33(2):397-410. doi: 10.1523/JNEUROSCI.0399-12.2013.

PMID: 23303920

 

Changes in Spontaneous firing patterns of cerebellar Purkinje cells in p75 knockout mice. 

Tian J, Tep C, Zhu MX, Yoon SO.

Cerebellum. 2013 Jun;12(3):300-3. doi: 10.1007/s12311-012-0439-6.

PMID: 23307658

2012

Macrophage migration inhibitory factor (MIF) is essential for inflammatory and neuropathic pain and enhances pain in response to stress.

Alexander JK, Cox GM, Tian JB, Zha AM, Wei P, Kigerl KA, Reddy MK, Dagia NM, Sielecki T, Zhu MX, Satoskar AR, McTigue DM, Whitacre CC, Popovich PG.

Exp Neurol. 2012 Aug;236(2):351-62. doi: 10.1016/j.expneurol.2012.04.018. Epub 2012 May 1.

PMID: 22575600

 

Structure and activity of the acid-sensing ion channels.

Sherwood TW, Frey EN, Askwith CC.

Am J Physiol Cell Physiol. 2012 Oct 1;303(7):C699-710. doi: 10.1152/ajpcell.00188.2012. Epub 2012 Jul 25.

PMID: 22843794

2011

Heteromeric acid-sensing ion channels (ASICs) composed of ASIC2b and ASIC1a display novel channel properties and contribute to acidosis-induced neuronal death.

Sherwood TW, Lee KG, Gormley MG, Askwith CC.

J Neurosci. 2011 Jun 29;31(26):9723-34. doi: 10.1523/JNEUROSCI.1665-11.2011.

PMID: 21715637

 

Rapid and efficient generation of functional motor neurons from human pluripotent stem cells using gene delivered transcription factor codes. 

Hester ME, Murtha MJ, Song S, Rao M, Miranda CJ, Meyer K, Tian J, Boulting G, Schaffer DV, Zhu MX, Pfaff SL, Gage FH, Kaspar BK.

Mol Ther. 2011 Oct;19(10):1905-12. doi: 10.1038/mt.2011.135. Epub 2011 Jul

PMID: 21772256

2010

Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN Nat Biotechnol.

Foust KD, Wang X, McGovern VL, Braun L, Bevan AK, Haidet AM, Le TT, Morales PR, Rich MM, Burghes AH, Kaspar BK.

Nat Biotechnol. 2010 Mar;28(3):271-4. doi: 10.1038/nbt.1610. Epub 2010 Feb 28.

PMID: 20190738

 

Activation of TRPA1 channels by fenamate nonsteroidal anti-inflammatory drugs.

Hu H, Tian J, Zhu Y, Wang C, Xiao R, Herz JM, Wood JD, Zhu MX.

Pflugers Arch. 2010 Mar;459(4):579-92. doi: 10.1007/s00424-009-0749-9. Epub 2009 Nov 4.

PMID: 19888597

 

TPCs: Endolysosomal channels for Ca2+ mobilization from acidic organelles triggered by NAADP.

FEBS Lett. 2010 May 17;584(10):1966-74. doi: 10.1016/j.febslet.2010.02.028. Epub 2010 Feb 14.

PMID: 20159015

2009

Sensorimotor enhancement in mouse mutants lacking the Purkinje cell-specific Gi/o modulator, Pcp2(L7).

Iscru E, Serinagaoglu Y, Schilling K, Tian J, Bowers-Kidder SL, Zhang R, Morgan JI, DeVries AC, Nelson RJ, Zhu MX, Oberdick J.

Mol Cell Neurosci. 2009 Jan;40(1):62-75. doi: 10.1016/j.mcn.2008.09.002. Epub 2008 Oct 1.

PMID: 18930827

 

Zinc activates damage-sensing TRPA1 ion channels. 

Hu H, Bandell M, Petrus MJ, Zhu MX, Patapoutian A.

Nat Chem Biol. 2009 Mar;5(3):183-90. doi: 10.1038/nchembio.146. Epub 2009 Feb 8.

PMID: 19202543

 

NAADP mobilizes calcium from acidic organelles through two-pore channels.

Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, Tang J, Rietdorf K, Teboul L, Chuang KT, Lin P, Xiao R, Wang C, Zhu Y, Lin Y, Wyatt CN, Parrington J, Ma J, Evans AM, Galione A, Zhu MX.

doi: 10.1038/nature08030.

 

Deletion of TRPC4 and TRPC6 in mice impairs smooth muscle contraction and intestinal motility in vivo. 

Tsvilovskyy VV, Zholos AV, Aberle T, Philipp SE, Dietrich A, Zhu MX, Birnbaumer L, Freichel M, Flockerzi V.

Gastroenterology. 2009 Oct;137(4):1415-24. doi: 10.1053/j.gastro.2009.06.046. Epub 2009 Jun 21.

PMID: 19549525  

 

TRPC5 is a Ca2+-activated channel functionally coupled to Ca2+-selective ion channels.

Gross SA, Guzmán GA, Wissenbach U, Philipp SE, Zhu MX, Bruns D, Cavalié A.

J Biol Chem. 2009 Dec 4;284(49):34423-32. doi: 10.1074/jbc.M109.018192. Epub 2009 Oct 8.

PMID: 19815560

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