Department of Neuroscience
Lab: 614-366-9409 or 614-366-9410
Current Research: A current research project studies the expression and function of two-pore domain K+ channels (K2Ps) in astrocytes. K2Ps are newly appreciated potassium channels that can interact with and be substantially modulated by a variety of physiochemical stimuli. Following the team’s recent observation of rat hippocampal astrocytes expressing K2P isoforms of TWIK-1 and TREK-1, the continuing research examines the unique features of K2Ps enabling astrocytes to maintain brain homeostatic function and subserve neuronal function with high efficiency. The ongoing projects address:
- The biophysical features of K2Ps of astrocytes in brain slices, as well as expression system
- Crosstalk of astrocyte K2Ps with neurotransmitters and its impact on astrocyte homeostatic function
- Interaction of K2Ps and gap junction channels, which in turn regulates the communication of astrocytes in their network
Astrocyte is the most populous cell type in the brain. The researchers seek to unveil the mysterious function of astrocytes and identifying glia-oriented therapeutic strategy for stroke treatment.
Research Techniques: This lab uses a variety of techniques to study the morphology and functional properties of astrocytes and other glial subtypes, such as NG2 glia, in the brain. Current projects include the use of the following preparations and techniques: acute brain slices, acute isolation of brain cells, confocal microscopy, Leica deconvolution microscopy, ion sensitive dye imaging, immunocytochemistry, RT-PCR, Western blots, single and dual patch clamp analysis. The lab also uses gene knockout mice to understand the functional role of K2Ps in astrocytes.
Degrees: Tongji Medical College, China, and Friedrich-Schiller-University Jena in Jena, Germany
Postdoctoral: Albany Medical College, Dr. Harold Kimelberg